师资队伍

魏娟

长聘教轨副教授

 

邮箱:wwwjuan@sjtu.edu.cn
主页:https://www.x-mol.com/groups/weijuan?lang=en

魏娟博士,上海交通大学药学院长聘教轨副教授、博士生导师。本科毕业于武汉大学,先后在上海交通大学阎超教授和英国华威大学Peter O’ Connor教授课题组,开展微分离分析方法开发和高分辨生物质谱技术的应用研究。回国前在波士顿大学生物药物质谱中心(Center for Biomedical Mass Spectrometry)担任研究科学家。
药物分析,生物多糖和糖蛋白研究
1. 高灵敏的精准分析方法开发
2. 生物多糖和糖蛋白药物分析
3. 基于蛋白质糖基化的生物标记物研究
1. Chen Z., Wei J., Tang Y., Lin C., Costello C. E., Hong P.. GlycoDeNovo2: An Improved MS/MS-Based De Novo Glycan Topology Reconstruction Algorithm. J Am Soc Mass Spectrom. 2022, 33(3):436-445
2. Wei, J., Tang Y, Ridgeway, M. E., Park, M. A., Costello, C. E., Lin, C. Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry Analytical Chemistry, 2020, 92 (19):13211-13220
3. Wei J., Tang Y., Bai, Y., Zaia, J., Costello, C. E., Lin, C. Toward Automatic and Comprehensive Glycan Characterization by Online PGC-LC-EED MS/MS. Analytical Chemistry, 2020, 92(1):782-791
4. Wei, J., Wu, J., Tang, Y., Ridgeway, M. E., Park, M. A., Costello, C. E., Zaia, J., Lin, C. Characterization and Quantification of Highly Sulfated Glycosaminoglycan Isomers by Gated-TIMS NETD MS/MS. Analytical Chemistry, 2019, 91(4), 2994-3001
5. Tang, Y.,‡ Wei, J.,‡ Costello, C. E., Lin, C. Characterization of Isomeric Glycans by Reversed Phase Liquid Chromatography-Electronic Excitation Dissociation Tandem Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 2018, 29(6):1295–1307.
6. Wei J., Antzutkin O. N., Barrow M. P., Dupree R., Brown S. P., O'Connor P. B. Amyloid Hydrogen Bonding Polymorphism Evaluated by 15N{17O}REAPDOR Solid-state NMR and Ultra-high Resolution FTICR-MS. Biochemistry, 2016, 55(14): 2065-2068
7. Wei J., Bristow A. W. T., O'Connor P. B. The Competitive Influence of Li+, Na+, K+, Ag+, and H+ on the Fragmentation of a PEGylated Polymeric Excipient. Journal of the American Society for Mass Spectrometry, 2015, 26: 166-173
8. Wei J, O'Connor P. B. Extensive Fragmentation of Pheophytin-a by Infrared Multiphoton Dissociation Tandem Mass Spectrometry. Rapid Communications in Mass Spectrometry, 2015, 29(24): 2411-2418
9. Wei J., Bristow A. W. T., McBride E., Kilgour D. P. A., O'Connor P. B. d-α-tocopheryl Polyethylene Glycol 1000 Succinate: A View from FTICR MS and Tandem MS. Analytical Chemistry, 2014, 86(3):1567-1574
10. Wei J., Li H. L., Barrow M., O’Connor P. B. Structural Characterization of Chlorophyll-a by High Resolution Tandem Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 2013, 24: 753-760
11. Wei J., Gu X., Wang Y., Wu Y, Yan C. Two-dimensional Separation System by on-line Hyphenation of Capillary Isoelectric Focusing with Pressurized Capillary Electrochromatography for Peptide and Protein Mapping. Electrophoresis, 2011, 32(2):230-237
12. Wei J., Gu X., Wang Y., Yan C. Off-line two-dimensional separation system cIEF/pCEC for peptides separation. Chinese Journal of Analytical Chemistry, 2011, 39(2):45-51
13. Wu, J., Wei, J., Hogan, J. D., Chopra, P., Joshi, A., Lu, W., Zaia, J. Negative Electron Transfer Dissociation Sequencing of 3-O-Sulfation-Containing Heparan Sulfate Oligosaccharides. Journal of the American Society for Mass Spectrometry, 2018, 29: 1262-1272
14. Wu, J., Wei, J., Hogan, J.D., Boons, G.J., Lin, C., Zaia, J. Sequencing Heparan Sulfate Using HILIC LC-NETD-MS/MS. Analytical Chemistry, 2019, 91(18): 11738-11746
15. Maqueda A. S., Xavier G-P, Wei J., Sze S. K. In-depth Analysis of Glutamine Deamidation by LERLIC-MS/MS in Shotgun Proteomics. Analytical Chemistry, 2016, 88(21): 10573-10582
16. Wu Y., Zhang X. H., Wei J., Xue Y., Bahatibieke M., Wang Y., Yan C. Recent advances and applications of CEC and pCEC. Chinese Journal of Chromatography, 2009, 27(5):609-620
17. Khatri, K., Pu, Y., Klein, J. A., Wei, J., Costello, C. E., Lin, C., Zaia, J. Comparison of Collisional and Electron-based Dissociation Modes for Middle-down Analysis of Multiply Glycosylated Peptides. Journal of the American Society for Mass Spectrometry, 2018, 29: 1075-1083
18. Lam, Y.P., Wootton, C.A., Hands-Portman, I., Wei, J., Chiu, C.K., Romero-Canelon, I., Lermyte, F., Barrow, M.P. and O’Connor, P.B. Does Deamidation of Islet Amyloid Polypeptide Accelerate Amyloid Fibril Formation? Chemical Communications, 2018, 54(98), 13853-13856
19. Lam, Y .P., Wootton, C. A., Hands-Portman, I., Wei, J., Chiu, C. K., Romero-Canelon, I., Lermyte, F., Barrow, M. P., and O’Connor, P. B. Determination of the Aggregate Binding Site of Amyloid Protofibrils Using Electron Capture Dissociation Tandem Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 2020, 5;31(2):267-276
20. van Agthoven M. A., Wootton C. A., Chiron L., Coutouly M. A., Soulby A., Wei J., Barrow M. P., Delsuc M-A, Christian-Rolando C., O’Connor P. B. Two-Dimensional Mass Spectrometry for Proteomics, a Comparative Study with Cytochrome C. Analytical Chemistry, 2016, 88(8): 4409-4417
21. van Agthoven M. A., Barrow M. P., Chiron L., Coutouly M. Wootton C. A., Wei J., A., Soulby A., Delsuc M-A, Christian-Rolando C., O’Connor P. B Differentiating Fragmentation Pathways of Cholesterol by Two-Dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 2015, 25: 2105-2114
22. Mei J., Tian Y. P., He W., Xiao Y. X., Wei J., Feng Y. Q. Preparation approaches of the coated capillaries with liposomes in capillary Electrophoresis. Journal of Chromatography A, 2010, 1217:6979-6986
1.开拓性的利用Omnitrap将基于电子激发裂解的多糖结构研究方法应用于离子轨道阱平台,在提高复杂多糖分析灵敏度的同时,结合导向性的多级串联质谱方法进一步推动深度多糖结构解析。(2020 ASMS, 大会口头报告)
2.首次在不依赖数据库的情况下,实现对复杂多糖混合物组份拓扑结构的自动解析。从色谱方法上的改进,到糖链连接的准确定性、 GlycoDenovo软件的辅助解析、以及对同分异构体糖型的相对定量,有效地填补了目前分析方法在生物多糖解析方面缺失的信息、推进蛋白质糖基化修饰和糖组学研究。(Anal. Chem. 2020, 92(1):782-791; J. Am. Soc. Mass Spectrom. 2018, 29(6):1295–1307;2019 ASMS, 大会口头报告)
3.开发色谱和离子淌度的分离新方法,结合碰撞诱导裂解和基于电子碎裂的串联质谱模式,对N-/O-糖、糖胺聚糖和糖肽等实现高分辨率分离和精细结构解析。(Anal. Chem. 2020, 92 (19):13211-13220;Anal. Chem. 2019, 91(4): 2994-3001; Anal. Chem. 2019, 91(18): 11738-11746等)
4.开发新型bottom-up蛋白质组学方法、top-down蛋白质分析流程、以及非标定量的糖蛋白质组学方法等,分别用于蛋白质脱酰胺作用、磷酸化修饰、糖基化修饰等翻译后修饰的研究,并应用于人体组织或血液中的生物标记物研究。(Anal. Chem. 2016, 88(21): 10573-10582;J. Am. Soc. Mass Spectrom.2018, 29: 1075-1083;Anal. Chem. 2016, 88(8): 4409-4417)
5.Amyloid-β 多肽和纤维的同位素精细结构分析以及纤维化过程研究,用于解析淀粉样纤维化的分子间相互作用和纤维化机理。(Biochemistry 2016, 55(14): 2065-2068;Chem. Comm. 2018, 54(98), 13853-13856;2014 ASMS, 大会口头报告;2014 BMSS, 大会口头报告)
6.利用高分辨质谱方法提升药物辅料混合物组成成分的定性定量分析。(AstraZeneca合作项目,Anal. Chem. 2014, 86(3):1567-1574;2013 ASMS, 大会口头报告)

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